The video, taken at Wyoming Premium Farms in Wheatland in April, shows employees abusing piglets and sows.
Tyson Foods initially said it had no connection to the farm, but then acknowledged it bought some sows from there.
The Humane Society's video, which was captured in just 27 days, showed pigs living in gestation cages that allow no room for the animals. Workers strike and hit the animals with their fists and kick full-grown pigs and piglets in the video.
The co-owner and manager of the farm, Doug DeRouchey, told 9NEWS the abuse would not be tolerated and he expected some employees to be fired over the video.
The Humane Society told 9NEWS that the Platte County, Wyo. Sheriff's Office is investigating the farm.